Methods of producing extracellular vesicles
AssigneeLonza Sales AG
InventorsRaymond Bourdeau, Su Chul Jang, Rane Harrison, Conlin O'Neil, Aaron Noyes
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Abstract
Provided herein are methods of preparing EVs, e.g., exosomes, associated with or encapsulated various cyclic dinucleotides, including STING agonists. Also provided herein are methods of loading EVs, e.g., exosomes, with various cyclic dinucleotides, including STING agonists.
Claims19
A method of preparing a composition comprising extracellular vesicles (EVs) associated with one or more cyclic dinucleotides (CDNs), comprising incubating the EVs with a loading concentration of cyclic dinucleotides (CDNs) in a mixture, wherein, after the incubation, the composition comprises EVs with loaded CDNs and free CDNs and wherein the free CDNs are removed by a multimodal chromatography.
A method of improving potency of cyclic dinucleotides (CDNs) in association with an EV, comprising:
- incubating the EV with the CDNs at a loading concentration of at least about 0.5 mM in a composition, wherein the composition, after the incubation, comprises the EVs with loaded CDNs and free CDNs, and
- removing the free CDNs from the EVs, wherein after the separation, the loaded CDNs are at a concentration between about 0.5 μM and about 10 μM, wherein the free CDNs are separated from the EVs by a multimodal chromatography.
Dependencies are inferred from the claim text.