DNA matrix processing method based on combined restriction digestion mechanism
AssigneeDalian University
InventorsQiang Zhang, Shihua Zhou, Shaoxia Xu, Yinan Hu, Bin Wang
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AssigneeDalian University
InventorsQiang Zhang, Shihua Zhou, Shaoxia Xu, Yinan Hu, Bin Wang
Estimating the term…
The present disclosure discloses a DNA matrix processing method based on a combined restriction digestion mechanism, including the following steps: constructing a single auxiliary strand-mediated combined restriction digestion mechanism; introducing an auxiliary strand based on the single auxiliary strand-mediated combined restriction digestion mechanism, to obtain a dual auxiliary strands-mediated combined restriction digestion mechanism; and constructing DNA matrix processing and a weighted sum of Boolean matrix multiplication with the dual auxiliary strands-mediated combined restriction digestion mechanism; in which the two auxiliary strands are directly used as elements involved in the matrix processing, and the 2N auxiliary strands are combined into N2 four-pronged restriction digestion structures in the presence of E6 type DNAzymes to cleave N2 substrate strands. Meanwhile, due to high-efficiency catalysis and specific recognition, the E6 type DNAzymes make the matrix processing rapid and accurate.
A DNA matrix processing method based on a combined restriction digestion mechanism, comprising the following steps:
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