Systems and methods for machine vision

A method, comprising:

• a) providing to an optical detection system comprising a detector:
• i) a first array of sites, wherein each individual site of the first array of sites comprises an individual optically-detectable moiety, wherein each individual site of the first array of sites is optically resolvable from each other site of the first array of sites, wherein a first subregion of the first array of sites has a signal occupancy of at least 50%, and wherein a second subregion of the first array of sites has a signal occupancy of no more than 10%;
• and
• ii) a second array of sites, wherein each individual site of the second array of sites is optically resolvable from each other site of the second array of sites, wherein each individual site of the second array of sites comprises one and only one individual analyte of a plurality of analytes immobilized to the second array of sites;
• b) detecting a first spatial arrangement of signals from optically-detectable moieties of the first subregion of the first array of sites;
• c) based upon the first spatial arrangement of signals from the first subregion of the first array of sites, determining a parameter of a detector model of the detector;
• d) after determining the parameter of the detector model, detecting a second spatial arrangement of signals from a first subregion of the second array of sites, and detecting a third spatial arrangement of signals from a second subregion of the second array of sites;
• and
• e) registering a signal of the second spatial arrangement of signals to a signal of the third spatial arrangement of signals, wherein the signal of the second spatial arrangement of signals and the signal of the third spatial arrangement of signals are emitted at a same site of the second array of sites.