Speckle-reduced illumination for improved scattering-based microscopy

Abstract

Speckle-modulated line illumination devices and associated methods are described that enable acquisition of images with high resolution and high speed simultaneously. One example speckle-modulated line illumination device includes a spatially coherent light source having a speckled output, a collimation lens positioned to receive the output of the spatially coherent light source, a cylindrical lens positioned to receive a collimated light produced by the collimation lens, and a diffuser positioned to receive a focused line illumination from the cylindrical lens, and to impart random phase variations in light that is output therefrom. The diffuser is coupled to a movement stage to impart rotational or translational movements to the diffusor as a function of time. Implementations of the disclosed technology can be used to develop reflectance confocal microscopy devices and scattering-based light sheet microscopy devices.

Dependencies are inferred from the claim text.