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AssigneeThe Regents of the University of California
InventorsMark van Zee, Dino Di Carlo, Joseph de Rutte, Sohyung Lee, Robert Damoiseaux, Cayden Williamson
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Degradable hollow shell particles are disclosed that can encapsulate cells within the hollow inner cavity that allows for the high-throughput screening and sorting of the encapsulated cells based on their phenotypic properties. The solid-phase of the particle is porous such that solution exchange can occur between the external environment and the interior cavity. Further, the solid-phase contains degradable crosslinkers and can be degraded to release enclosed biological entities. An example embodiment consists of encapsulating a cell within the hollow shell particle, allowing the cell to accumulate biomass, selecting hollow shell particles based on accumulated biomass, and degrading the hollow shell particles to release the cells and develop hyper-producing cell lines. Exemplary cell types include microalgae, mammalian cells, bacteria, yeast, and fungi.
A method of using a particle system comprising a plurality of hollow shell particles formed from a biocompatible and chemically or physically degradable crosslinked hydrogel, each hollow shell particle having a void or cavity formed therein and surrounded by a shell of crosslinked hydrogel, and one or more live cells contained in the void or cavity, the method comprising incubating the plurality of hollow shell particles in a growth medium for a period of time to obtain multicellular colonies contained in at least some of the plurality of hollow shell particles followed by subjecting the plurality of hollow shell particles to sorting by flow cytometry, wherein the sorting operation sorts hollow shell particles based on accumulated biomass of the multicellular colonies contained in respective hollow shell particles using a forward and/or side scatter signal obtained during flow cytometry.
A method of using a particle system comprising a plurality of hollow shell particles formed from a biocompatible and chemically or physically degradable crosslinked hydrogel, each hollow shell particle having a void or cavity formed therein and surrounded by a shell of crosslinked hydrogel, and one or more live cells contained in the void or cavity, the method, comprising:
A method of using a particle system comprising:
Dependencies are inferred from the claim text.