Multiplex detection of vulvovaginal candidiasis, trichomoniasis and bacterial vaginosis
AssigneeAugusta SpinCo Corporation
InventorsNancy Paquette, Marie-Helene Tremblay, Simon Tremblay, Roseline Therrien, Marie-Christine Fortin, Lucile Belley-Montfort, Dany Cantin, Celine Roger-Dalbert
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Abstract
Methods and compositions for detection of vulvovaginal candidiasis (VVC), trichomoniasis and bacterial vaginosis (BV) are disclosed herein. In some embodiments, the presence or absence of VVC-associated Candida, Trichomonas valginalis, and a plurality of BV-related bacteria in a sample is determined using multiplex nucleic acid-based testing methods.
Claims14
A kit for detecting a bacterial vaginosis (BV)-related bacteria in a biological sample, wherein the BV-related bacteria is selected from the group consisting of:
- Lactobacillus crispatus, Lactobacillus jensenii, Gardnerella vaginalis, Atopobium vaginae, Megasphaera Type 1, BVAB2, and a combination thereof, the kit comprising:
- (a) at least one pair of primers capable of hybridizing to the 16S rRNA gene of Atopobium vaginae, wherein each primer in said at least one pair of primers comprises a sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, or sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 1 or SEQ ID NO:
- 2;
- and
- an oligonucleotide probe comprising a sequence of SEQ ID NO:
- 3, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 3,
- (b) at least one pair of primers capable of hybridizing to the 16S rRNA gene of BVAB2, wherein each primer in said at least one pair of primers comprises a sequence of SEQ ID NO:
- 4 or SEQ ID NO:
- 5, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 4 or SEQ ID NO:
- 5;
- and
- an oligonucleotide probe comprising a sequence of SEQ ID NO:
- 6, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 6,
- (c) at least one pair of primers capable of hybridizing to the 16S rRNA gene of Megasphaera type 1, wherein each primer in said at least one pair of primers comprises a sequence of SEQ ID NO:
- 7 or SEQ ID NO:
- 8, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 7 or SEQ ID NO:
- 8;
- and
- an oligonucleotide probe comprising a sequence of SEQ ID NO:
- 9, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 9,
- (d) at least one pair of primers capable of hybridizing to the vly gene of Gardnerella vaginalis, wherein each primer in said at least one pair of primers comprises a sequence selected from the group consisting of SEQ ID NOS:
- 10-12, or a sequence differing by only one nucleotide mismatch relative to a sequence selected from the group consisting of SEQ ID NOS:
- 10-12;
- and
- an oligonucleotide probe comprising a sequence of SEQ ID NO:
- 13, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 13, and
- (e) at least one pair of primers capable of hybridizing to the 16S rRNA genes of Lactobacillus crispatus and Lactobacillus jensenii, wherein each primer in said at least one pair of primers comprises a sequence of SEQ ID NO:
- 14 or SEQ ID NO:
- 15, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 14 or SEQ ID NO:
- 15;
- and
- an oligonucleotide probe comprising a sequence of SEQ ID NO:
- 16, or a sequence differing by only one nucleotide mismatch relative to SEQ ID NO:
- 16,
- wherein each of the oligonucleotide probes of (a), (b), (c), (d) and (e) comprises a detectable moiety selected from the group consisting of a fluorescence emitter moiety, a fluorescence quencher moiety, a fluorophore, biotin, radionucleotides, radioactive label, and a chemiluminescent agent.
Dependencies are inferred from the claim text.