Method and device for fixed-point editing of nucleotide sequence with stored data

A method for fixed-point editing of a nucleic acid sequence with stored data, which comprises the following steps:

• (1) splitting a nucleic acid sequence by which data is stored into a plurality of sequence fragments, and dividing all the sequence fragments into i partitions, wherein i is a positive integer;
• (2) adding a theoretical partition adapter at one or both ends of the sequence fragments in each partition, wherein sequence of the partition adapter for each partition is different from each other;
• (3) synthesizing the sequence fragments in each partition as described in step (2) to obtain nucleic acid fragments;
• (4) determine a partition n where a sequence fragment to be edited is located, and record the partition as the nth partition;
• (5) amplifying sequence fragments of all partitions except for sequence fragments of the nth partition by using a partition primer library, wherein the partition primer library comprises primers that are at least partially complementary to sequences of the partition adapter for all partitions except for the nth partition, so as to obtain a library comprising the sequence fragments of all partitions except for the sequence fragments of the nth partition;
• and
• (6) correcting a wrong sequence in the sequence fragment to be edited recited in step (4) in the nth partition to obtain a correct sequence, then synthesizing all sequence fragments in the nth partition according to the correct sequence, and adding them into the library of step (5) so as to obtain a library with the correct sequence.