Back to results
US 12,723,267 B2Granted
Method for inducing targeted meiotic recombinations
AssigneesMeiogenix, Institut Curie, Centre National De La Recherche Scientifique, Sorbonne Universite
InventorsGiacomo Bastianelli, Alexandre Serero, Alain Nicolas
Life of this patent
Estimating the term…
Abstract
The present invention relates to a fusion protein comprising a Cas9 domain and a Spo11 domain, as well as the use of this protein to induce targeted meiotic recombinations in a eukaryotic cell.
Claims33
A method for inducing targeted meiotic recombination in a plant cell comprising:
- expressing in the plant cell:
- a) a fusion protein comprising a bacterial Cas9 protein lacking nuclease activity and a Spo11 protein;
- and
- b) one or more guide RNAs, the guide RNAs comprising an RNA structure for binding to the Cas9 protein of the fusion protein and a sequence complementary to a targeted chromosomal region;
- thereby inducing meiotic recombination at the targeted chromosomal region,
- wherein the meiotic recombination at the targeted chromosomal region is increased as compared to the meiotic recombination at the targeted chromosomal region in a control plant cell lacking the fusion protein or the one or more guide RNAs.
3 more elements
Dependencies are inferred from the claim text.