Root-knot nematode disease-related miRNA and its regulatory gene, protein and application
AssigneeJiangsu Academy of Agricultural Sciences
InventorsDongmei Zhou, Lihui Wei, Chunting Wang
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Abstract
The present application provides a root-knot nematode disease-related miRNA and its regulatory gene, proteins and application. The miRNA is miRcn1, and the genes regulated by miRcn1 include CRF9. The protein is encoded by the root-knot nematode disease-related miRNA regulatory gene provided by the present application. The application includes resistance to root-knot nematode disease. The present application experimentally confirms that miRNA miRcn1 and its target gene CRF9 have an important role in the control of root-knot nematodes. Overexpression of miRNA miRcn1 and silencing of the target gene CRF9 can improve plant resistance to root-knot nematodes.
Claims2
An application of an isolated miRNA in the prevention and/or control of root-knot nematode disease, wherein the isolated miRNA is miRcn1 shown in SEQ ID NO:
- 1;
- and, miRcn1 inhibits the expression of CRF9 gene, wherein the prevention and/or treatment of root-knot nematode disease comprises:
- preventing and/or treating root-knot nematode infections occurring in plant;
- or
- enhancing resistance to root-knot nematodes of plant;
- wherein the plant is the tomato plant overexpressing CRF9 gene.
A method of producing transgenic plant, wherein the method includes the following steps:
- S1, transforming an isolated miRNA into plant callus;
- and,
- S2, regenerating a transgenic plant from the plant callus obtained in S1;
- wherein the isolated miRNA is miRcn1 shown in SEQ ID NO:
- 1;
- miRcn1 inhibits the expression of CRF9 gene;
- and, the plant is a tomato plant.
Dependencies are inferred from the claim text.