Resistance gene TaCAT to Fusarium crown rot of wheat and application thereof

A method for detecting an allelotype with resistance or susceptibility to Fusarium crown rot in wheat, comprising:

• extracting genomic DNA of a to-be-detected wheat;
• taking the genomic DNA of the to-be-detected wheat as a template, performing a polymerase chain reaction (PCR) amplification with an upstream primer with the nucleotide sequence as shown in SEQ ID NO:
• 5, and a downstream primer with the nucleotide sequence as shown in SEQ ID NO:
• 6 to obtain an amplification product, comparing a gene sequence of the amplification product with the gene sequence as shown in SEQ ID NO:
• 1 to obtain a comparison result, and determining an allelotype of the to-be-detected wheat based on the comparison result;
• wherein the determining an allelotype of the to-be-detected wheat based on the comparison result comprises:
• in response to 215 base pairs (bp) in the gene sequence of the amplification product being missing starting from a 654th position after a start codon adenine-thymine-guanine (ATG) compared to the gene sequence as shown in SEQ ID NO:
• 1, determining that the to-be-detected wheat is a deletion type;
• and
• in response to the gene sequence of the amplification product being the same as the nucleotide sequence as shown in SEQ ID NO:
• 1, determining that the to-be-detected wheat is an insertion type;
• determining that the to-be-detected wheat is resistant to Fusarium crown rot when the allelotype of the to-be-detected wheat is determined to be the deletion type;
• and
• breeding a wheat variety resistant to Fusarium crown rot by using the to-be-detected wheat determined to be resistant.