System and method for combating plant pathogenic bacterial infections

A system for combating infections due to a plant pathogen, the system comprises:

• a sample collection module for obtaining a sample from an infected area;
• a pathogen detection and DNA extraction module isolating DNA from the obtained sample using one of a laboratory methods;
• a sequencer for sequencing the isolated DNA;
• one or more hardware processors;
• a memory in communication with the one or more hardware processors, wherein the one or more first hardware processors are configured to execute programmed instructions stored in the one or more first memories, to:
• identify a set of nucleotide repeat sequences in the sequenced DNA which occur more than a predefined number of times in the plant pathogen;
• identify a set of neighborhood genes present upstream and downstream of the set of nucleotide repeat sequences;
• annotate the set of neighborhood genes according to their functional roles in their respective pathogen based on their involvement in pathways in the identified set of neighborhood genes;
• and
• test presence of a secondary structure in the identified set of nucleotide repeat sequences;
• an administration module for preparing and administering an engineered polynucleotide construct on the infected plant to combat the infections due to the plant pathogen, wherein the engineered polynucleotide construct is comprising:
• one or more of the set of nucleotide repeat sequences with multiple copies dispersed in nucleotide sequences of genomes of Pseudomonas syringae or Xanthomonas axonopodis, wherein the set of nucleotide repeat sequences comprises one or more of a Sequence ID 001, reverse complement of the Sequence ID 001, Sequence ID 002, reverse complement of the Sequence ID 002,
• a first enzyme capable of nicking and cleaving the identified set of nucleotide repeat sequences, and
• a second enzyme capable of removal of a set of neighborhood genes flanking the set of nucleotide repeat sequences;
• and
• an efficacy module for
• check the efficacy of the administered engineered polynucleotide construct to combat the plant pathogen after a predefined time period, and
• re-administer the engineered polynucleotide construct if the plant pathogen is still present in the infected plant post administering.