HD-ZIP transcription factor ArHDZ19 of Anoectochilus roxburghii Hongxia, encoding gene and use of encoding gene
AssigneeZhejiang A & F University
InventorsQingsong Shao, Yu Zhang, Taowei Zhong, Aimin Lv
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Abstract
An HD-ZIP transcription factor ArHDZ19 of Anoectochilus roxburghii ‘Hongxia’, encoding gene, and use of encoding gene are provided. The transcription factor ArHDZ19 includes a polypeptide having the amino acid sequence as shown in SEQ ID NO: 2. A gene for encoding the above-mentioned proteins having the nucleotide sequence as shown in SEQ ID NO: 1 is provided. The present invention provides a theoretical basis for using genetic engineering technology to promote the reproductive growth of Anoectochilus roxburghii ‘Hongxia’ and improve seed vitality, and has important application value.
Claims6
A transgenic plant, comprising a recombinant expression vector, wherein the recombinant expression vector comprises an encoding gene for HD-ZIP transcription factor ArHDZ19 of Anoectochilus roxburghii ‘Hongxia’, wherein the nucleotide sequence for the encoding gene is SEQ ID NO:
- 1 and an amino acid sequence of the encoded HD-ZIP transcription factor ArHDZ19 is SEQ ID NO:
- 2.
A method for promoting reproductive growth of the Anoectochilus roxburghii ‘Hongxia’, comprising:
- (a) constructing a recombinant expression vector pHB-ArHDZ19 comprising the nucleotide sequence of SEQ ID NO:
- 1 encoding the HD-ZIP transcription factor ArHDZ19 having the amino acid sequence of SEQ ID NO:
- 2;
- (b) transforming Anoectochilus roxburghii ‘Hongxia’ with the recombinant expression vector;
- (c) cultivating the transformed Anoectochilus roxburghii ‘Hongxia’;
- and
- (d) screening and selecting transgenic Anoectochilus roxburghii ‘Hongxia’ plants that comprise the recombinant expression vector or an integrated nucleic acid comprising the nucleotide sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.