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US 12,723,253 B2Granted
Use of MdPHY7 protein or MdPHY7 gene of apple in regulating anthocyanin biosynthesis
AssigneeNanjing Hejiachun Biological Technology Co., Ltd.
InventorsLiangju Wang, Liuzi Zhang, Jianting Zhang
Life of this patent
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Abstract
Disclosed is use of an MdPHY7 protein or an MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of the apple anthocyanin biosynthesis, belonging to the technical field of plant genetic engineering. Exogenous 5-aminolevulinic acid (ALA) treatment can induce expression of the MdPHY7 gene, thereby promoting an increase of the apple anthocyanin biosynthesis, and then ultimately resulting in accumulation of apple anthocyanins to promote apple fruit coloring.
Claims2
A method for inhibiting apple anthocyanin biosynthesis, comprising the following steps:
- infecting an apple tissue with a recombinant Agrobacterium strain carrying an interfering recombinant plasmid;
- wherein the interfering recombinant plasmid is inserted with an apple anthocyanin biosynthesis-related MdPHY7 gene fragment having the nucleotide sequence set forth in SEQ ID NO:
- 3;
- and
- wherein a backbone plasmid of the interfering recombinant plasmid is an RNA interference vector pHELLSGATE4;
- and an insertion site of the MdPHY7 gene fragment on the backbone plasmid is between attP1 and attP2.
3 more elements
Dependencies are inferred from the claim text.