Materials and methods for increasing the tocopherol content in seed oil

A method for producing a Brassica napus oil comprising n-3 very long chain polyunsaturated fatty acid (VLC-PUFA) and tocopherol, comprising:

• (i) providing transgenic Brassica napus seeds comprising a biosynthetic pathway that synthesizes eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), wherein each transgenic seed comprises multiple expression cassettes comprising transgenes which comprise at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase;
• and
• (ii) obtaining seed oil from the transgenic seeds, wherein said seed oil comprises increased tocopherol content and increased n-3 very long chain polyunsaturated fatty acid (VLC-PUFA) content as compared to control Brassica napus seeds, wherein the increased n-3 VLC-PUFA content comprises EPA, DPA, and/or DHA,
• wherein the increased tocopherol content and the increased n3 VLC-PUFA content in the transgenic seeds are positively correlated, and
• wherein the increased tocopherol content comprises increased total tocopherol content, and the increased total tocopherol content is the result of the biosynthetic pathway that synthesizes EPA, DPA, and DHA.