Materials and methods for PUFA production, and PUFA-containing compositions

A plant or seed thereof of family Brassicaceae with a genotype that confers a heritable phenotype of seed oil very long chain polyunsaturated fatty acid (VLC-PUFA) content, obtained from progeny lines prepared by a method comprising the steps of

• i) crossing a plant of family Brassicaceae, said plant comprising a T-DNA comprising one or more expression cassettes encoding for one or more delta-5 desaturase (d5Des), one or more delta-6 elongase (d6Elo), one or more delta-6 desaturase (d6Des), one or more omega 3 desaturase (o3Des), one or more delta-5 elongase (d5Elo) and two or more different delta-4 desaturases (D4Des), with a plant of family Brassicaceae, said plant not comprising said T-DNA, to yield a F1 hybrid,
• ii) selfing the F1 hybrid for at least one generation, and
• iii) selecting the progeny of step (ii) comprising the T-DNA and which produce seed comprising VLC-PUFA such that the content of all VLC-PUFA downstream of 18:1n-9 is at least 40% (w/w) of the total seed fatty acid content at an oil content of 40% (w/w) and such that the content of eicosapentaenoic acid (EPA) is at least 6% (w/w) and the content of docosahexaenoic acid (DHA) is at least 0.8% (w/w) of the total seed fatty acid content at an oil content of 40% (w/w).