Method for obtaining endothelial cells from pluripotent stem cells

A method for differentiating pluripotent stem cells into endothelial cells, comprising the steps of:

• a) on day 0, the pluripotent stem cells are dissociated, seeded at a density of 40 000 to 60 000 cells/cm2 on a matrix and cultured in monolayer conditions in the presence of a medium suitable for the culture of pluripotent cells, further comprising fibroblast growth factor 2 (FGF2) and a ROCK inhibitor;
• b) on day 1, the medium is replaced with a medium suitable for mesoderm induction, further comprising a GSK3 (Glycogen Synthase Kinase 3) inhibitor and BMP4 (Bone Morphogenetic Protein 4);
• c) on day 4, the medium is replaced with a medium suitable for the culture of endothelial cells, further comprising VEGF (Vascular Endothelial Growth Factor) and forskolin;
• d) on day 6, the cells are dissociated and selected for expression of the CD144 marker,
• wherein said process meets good manufacturing practices (GMP) standards.