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US 12,723,236 B2Granted
Method for producing cell aggregate
AssigneesToppan Holdings Inc., Osaka University
InventorsYasuyuki Hiraoka, Michiya Matsusaki
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Abstract
Disclosed is a method for producing a cell aggregate having bile canaliculi, including a contact step of bringing cells including hepatocytes into contact with heparin in an aqueous medium and a culture step of culturing the cells brought in contact with the heparin to form the cell aggregate. A concentration of heparin in the aqueous medium when it is brought into contact with the cells may be 0.5 mg/mL or more.
Claims8
A method for producing a cell aggregate comprising bile canaliculi, comprising:
- a contact step of bringing cells comprising hepatocytes into contact with heparin in an aqueous medium;
- and
- a culture step of culturing the cells brought in contact with the heparin to form the cell aggregate,
- wherein a concentration of heparin in the aqueous medium when brought into contact with the cells is 0.5 mg/mL or more.
1 more element
Dependencies are inferred from the claim text.