Method for preparing skin-derived pluripotent precursor cells
AssigneesKao Corporation, Osaka University
InventorsYoriko Nakagiri, Kiyotoshi Sekiguchi
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Abstract
The present invention relates to improvement of the yield of skin-derived pluripotent precursor cells in induction of differentiation of stem cells to skin-derived pluripotent precursor cells. The present invention provides a method for preparing skin-derived pluripotent precursor cell comprising culturing a neural crest stem cells in the presence of at least one selected from the group consisting of laminin and a fragment thereof to differentiate the cells to skin-derived pluripotent precursor cells, wherein the laminin is at least one selected from the group consisting of laminin 111, laminin 121, laminin 332, laminin 421, laminin 511, laminin 521, and a variant thereof.
Claims10
A method for preparing skin-derived pluripotent precursor cells, the method comprising:
- culturing neural crest stem cells in the presence of a perlecan-modified laminin 111, a perlecan-modified laminin 121, a perlecan-modified laminin 332, a perlecan-modified laminin 421, a perlecan-modified laminin 511, a perlecan-modified laminin 521, or a combination thereof to differentiate the cells to skin-derived pluripotent precursor cells.
Dependencies are inferred from the claim text.