Systems and methods for automated cell culturing
AssigneeMytos Bio Limited
InventorsAli Afshar, James Cunningham, Henry Miskin, Xian Weng Jiang, Ignacio Willats, Sina Atashpazgargari, Benedict Greenberg
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Abstract
Systems and methods for automated cell culturing are disclosed. In some embodiments, one or more cell culture vessels are fluidly connected with one or more multiport valves and one or more fluid pumps. The fluid pumps may pump various fluids into and out of the cell culture vessels as necessary to support cell growth, routed by the one or more multiport valves. In some embodiments, one or more components may be removable from other components so that some components may be prepared and sterilized independently prior to usage.
Claims10
A method of seeding a cell sample into a cell culture container, comprising:
- coupling a cell culture tray assembly to a support plate of an instrument,
- the cell culture tray assembly including a tray, the cell culture container coupled to the tray, a pump, and a valve assembly removably coupled to the tray, the cell culture container aseptically coupled to the pump and the valve assembly to form a closed system, the valve assembly and the pump each configured to be actuated to cause transfer of a fluid into or out of the cell culture container;
- the instrument including the support plate, a valve actuator, a pump actuator, and an agitator assembly, the agitator assembly configured to agitate the support plate and includes a plurality of rotatable coupling elements, each of the plurality of rotatable coupling elements coupled to a corresponding attachment location from a plurality of attachment locations on the support plate to maintain a position of the support plate relative to the instrument in at least two directions;
- a bottom surface of the support plate includes a magnet at each attachment location from the plurality of attachment locations that are magnetically coupled to a ferromagnetic portion of each rotatable coupling element from the plurality of rotatable coupling elements to maintain the position of the support plate relative to the instrument in at least a vertical direction;
- coupling a seeding container within the closed system to the container, the pump, and the valve assembly, the seeding container containing the cell sample;
- actuating at least one of the pump or the valve assembly to convey a portion of the cell sample from the seeding container to the cell culture container to seed the cell culture container with the cell sample;
- and
- actuating the agitator assembly to agitate the support plate and the cell culture tray assembly while the portion of the cell sample is being conveyed from the seeding container into the cell culture container.
Dependencies are inferred from the claim text.