Single-cell screening device, screening assembly, screening method, and use thereof

A single-cell screening device, comprising:

• a body, comprising a first surface, and a second surface arranged opposite to the first surface;
• a plurality of spray holes, disposed in the body, wherein the spray holes extend from openings on the second surface and extend toward the first surface without penetrating the first surface;
• heating portions, disposed in the spray holes and connected to top surfaces of the spray holes, the heating portions being used to heat liquid entering the spray holes to produce bubbles, wherein the bubbles eject cells entering the spray holes;
• and
• a flow channel, disposed in the body, the flow channel extends from an opening from the first surface and extends to the spray holes according to a predetermined path, to deliver a cell suspension into the spray holes;
• wherein the flow channel includes a main flow channel and a plurality of sub-flow channels connected to the main flow channel, each of the spray holes being connected to one of the sub-flow channels;
• wherein the main flow channel includes a column array for lining up cells before the cell suspension enters the sub-flow channels.