Device and method for the delivery of molecules into cells

A device for delivery of molecules into the interior of cells, the device comprising:

• a heat source configured to heat a tissue of interest comprising a plurality of cells to an elevated temperature between 40° C. and 43° C. for a duration of between 2 minutes and 5 minutes;
• an applicator for directing a stream of charged gas particles at the tissue of interest that has been heated to the elevated temperature to deliver one or more molecules into an interior of one or more of the plurality of cells, wherein the applicator is not in contact with the tissue of interest, the applicator comprising;
• a hollow body having a first and a second end;
• a gas source in fluid communication with the first end of the hollow body, wherein the gas source supplies a flow of gas through the hollow body;
• and
• an electrode secured to the second end of the hollow body, wherein the electrode is connected to a power supply to generate an electric field and to ionize gas from the gas source, wherein the electric field is created using a constant direct current (DC) voltage.