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AssigneeThe University of British Columbia
InventorsBlair Leavitt, Pieter Cullis, Terri Petkau, Austin Hill, Pamela Wagner, Jayesh Kulkarni
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Lipid-based vesicles, typically herein called transfection competent vesicles (TCVs), configured to safely and efficiently deliver DNA, RNA, other nucleic acid and protein cargoes into target cells. The safety and efficiency are each, and both, achieved in part by eliminating organic solvents such as ethanol and detergents such as sodium dodecyl sulfate from the TCV loading processes (i.e., inserting a cargo into the TCV), TCV storage processes, and/or TCV delivery processes. The cargoes can also comprise nucleic acids complexed with a protein, such as a ribonucleoprotein (RNP). The systems, compositions, devices and methods, etc., herein, in some embodiments, can provide empty TCVs that can if desired be loaded at the bench without use of specialized equipment.
A method of transfection, the method comprising administering to a patient a composition comprising a water-based solution which comprises:
A method of transfection, the method comprising transfecting a target cell with a composition comprising a water-based solution which comprises:
A method for treating Huntington's disease in a patient, the method comprising administering to the brain of the patient a therapeutically effective amount of a composition comprising a water-based solution which comprises:
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