Antigen-binding protein targeting CD70 and use thereof
OwnerWenbo Wang, Yanni Lininventors
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Abstract
An antigen-binding protein targeting CD70. In particular, the present invention relates to a chimeric antigen receptor containing the antigen binding protein and the use thereof, and cells containing or expressing the antigen binding protein. The antigen-binding protein binds to a CD70 protein at 1 nM of a KD value. The chimeric antigen receptor containing the antigen-binding protein has strong specific recognition and binding abilities to the CD70 protein. The cells containing or expressing the antigen-binding protein can secrete cytokines IL-2 and IFN-γ.
Claims49
A chimeric antigen receptor, containing a targeting moiety, wherein the targeting moiety contains a heavy chain complementary determining region 1 (HCDR1), a heavy chain complementary determining region 2 (HCDR2) and a heavy chain complementary determining region 3 (HCDR3), wherein the HCDR1, HCDR2 and HCDR3 comprise amino acid sequences selected from the groups consisting of:
- a) HCDR1:
- SEQ ID NO:
- 1, HCDR2:
- SEQ ID NO:
- 2, and HCDR3:
- SEQ ID NO:
- 3;
- b) HCDR1:
- SEQ ID NO:
- 1, HCDR2:
- SEQ ID NO:
- 2, and HCDR3:
- SEQ ID NO:
- 4;
- c) HCDR1:
- SEQ ID NO:
- 1, HCDR2:
- SEQ ID NO:
- 2, and HCDR3:
- SEQ ID NO:
- 5;
- d) HCDR1:
- SEQ ID NO:
- 6, HCDR2:
- SEQ ID NO:
- 7, and HCDR3:
- SEQ ID NO:
- 8;
- e) HCDR1:
- SEQ ID NO:
- 9, HCDR2:
- SEQ ID NO:
- 7, and HCDR3:
- SEQ ID NO:
- 10;
- f) HCDR1:
- SEQ ID NO:
- 11, HCDR2;
- SEQ ID NO:
- 12, and HCDR3:
- SEQ ID NO:
- 13;
- g) HCDR1:
- SEQ ID NO:
- 14, HCDR2:
- SEQ ID NO:
- 15, and HCDR3:
- SEQ ID NO:
- 16;
- h) HCDR1:
- SEQ ID NO:
- 17, HCDR2:
- SEQ ID NO:
- 18, and HCDR3:
- SEQ ID NO:
- 19;
- i) HCDR1:
- SEQ ID NO:
- 20, HCDR2:
- SEQ ID NO:
- 21, and HCDR3:
- SEQ ID NO:
- 22;
- i) HCDR1:
- SEQ ID NO:
- 23, HCDR2:
- SEQ ID NO:
- 24, and HCDR3:
- SEQ ID NO:
- 25;
- k) HCDR1:
- SEQ ID NO:
- 26, HCDR2:
- SEQ ID NO:
- 27, and HCDR3:
- SEQ ID NO:
- 28;
- l) HCDR1:
- SEQ ID NO:
- 29, HCDR2:
- SEQ ID NO:
- 30, and HCDR3:
- SEQ ID NO:
- 31;
- and m) HCDR1:
- SEQ ID NO:
- 23, HCDR2:
- SEQ ID NO:
- 32, and HCDR3:
- SEQ ID NO:
- 33.
An isolated antigen-binding protein specifically binding to CD70 at 1 nM or less of a KD value, wherein the isolated antigen-binding protein comprises a heavy chain complementary determining region 1 (HCDR1), a heavy chain complementary determining region 2 (HCDR2) and a heavy chain complementary determining region 3 (HCDR3), wherein the HCDR1, HCDR2 and HCDR3 comprise amino acid sequences selected from the groups consisting of:
- a) HCDR1:
- SEQ ID NO:
- 1, HCDR2:
- SEQ ID NO:
- 2, and HCDR3:
- SEQ ID NO:
- 3;
- b) HCDR1:
- SEQ ID NO:
- 1, HCDR2:
- SEQ ID NO:
- 2, and HCDR3:
- SEQ ID NO:
- 4;
- c) HCDR1:
- SEQ ID NO:
- 1, HCDR2:
- SEQ ID NO:
- 2, and HCDR3:
- SEQ ID NO:
- 5;
- d) HCDR1:
- SEQ ID NO:
- 6, HCDR2:
- SEQ ID NO:
- 7, and HCDR3:
- SEQ ID NO:
- 8;
- e) HCDR1:
- SEQ ID NO:
- 9, HCDR2:
- SEQ ID NO:
- 7, and HCDR3:
- SEQ ID NO:
- 10;
- f) HCDR1:
- SEQ ID NO:
- 11, HCDR2:
- SEQ ID NO:
- 12, and HCDR3:
- SEQ ID NO:
- 13;
- HCDR1:
- SEQ ID NO:
- 14, HCDR2:
- SEQ ID NO:
- 15, and HCDR3:
- SEQ ID NO:
- 16;
- h) HCDR1:
- SEQ ID NO:
- 17, HCDR2:
- SEQ ID NO:
- 18, and HCDR3:
- SEQ ID NO:
- 19;
- i) HCDR1:
- SEQ ID NO:
- 20, HCDR2:
- SEQ ID NO:
- 21, and HCDR3:
- SEQ ID NO:
- 22;
- i) HCDR1:
- SEQ ID NO:
- 23, HCDR2:
- SEQ ID NO:
- 24, and HCDR3:
- SEQ ID NO:
- 25;
- k) HCDR1:
- SEQ ID NO:
- 26, HCDR2:
- SEQ ID NO:
- 27, and HCDR3:
- SEQ ID NO:
- 28;
- l) HCDR1:
- SEQ ID NO:
- 29, HCDR2:
- SEQ ID NO:
- 30, and HCDR3:
- SEQ ID NO:
- 31;
- and m) HCDR1:
- SEQ ID NO:
- 23, HCDR2:
- SEQ ID NO:
- 32, and HCDR3:
- SEQ ID NO:
- 33.
Dependencies are inferred from the claim text.