Back to results
US 12,721,815 B2Granted
Freeze-dried exosome composition and uses thereof
AssigneeAVECIN-Biopharma Oy
InventorSepideh Parvanian
Life of this patent
Estimating the term…
Abstract
The invention relates to compositions and methods for exosome preservation by freeze-drying as well as methods of use of the freeze-dried exosome compositions.
Claims19
A cryoprotectant for exosomes comprising trehalose and low molecular weight hyaluronic acid (LMW HA) having molecular weight lower than 10 kDa wherein:
- the w/w ratio of trehalose to low molecular weight hyaluronic acid is between 0.3 and 0.7, the concentration in the combined exosome cryoprotectant preparation of trehalose is at least 25 mM and/or at most 100 mM, and/or
- the concentration in the combined exosome cryoprotectant preparation of LMW HA is at least 1% (w/v) and/or at most 5% (w/v),
- which provides enhanced exosome stability and bioactivity after storage, of a smaller homogeneous, and higher concentration of exosomes compared to LMW HA and trehalose alone.
A freeze-dried composition comprising exosomes, low molecular weight hyaluronic acid (LMW HA) having molecular weight lower than 10 kDa and trehalose wherein:
- the w/w ratio of trehalose to low molecular weight hyaluronic acid is between 0.3 and 0.7, the concentration in the combined exosome cryoprotectant preparation of trehalose is at least 25 mM and/or at most 100 mM, and/or
- the concentration in the combined exosome cryoprotectant preparation of LMW HA is at least 1% (w/v) and/or at most 5% (w/v),
- which provides enhanced exosome stability and bioactivity after storage, of a smaller homogeneous, and higher concentration of exosomes compared to LMW HA and trehalose alone.
Dependencies are inferred from the claim text.