Microbial formulation for the protection of plants and agricultural crops against environmental conditions and methods of manufacture and use thereof

A composition for the protection of agricultural plants and crops against unfavorable environmental conditions for their growth, wherein the composition comprises:

• a. a micro-organism without ice-nucleating activity (NINA) isolated and identified from plants inhabiting different extreme environments, including the Antarctic territory, capable of growing in the presence of carbohydrates and/or carbon-based substrate, and is resistant to environmental stress, including water stress and cold stress;
• wherein the microorganism is selected from the list comprising:
• microorganisms of the rhizosphere resistant to extreme conditions without ice nucleating activity and without IBP genes, NINA bacteria, recombinant microorganisms without ice nucleating activity, isolated strains of NINA bacteria, strains of Pseudomonas NINA, including the GPI-1 strain with a 16S rDNA of SEO ID NO. 1, and any other microorganism without ice-nucleating activity isolated from a rhizosphere sample of plants of different species, including environment under extreme conditions, and where the selected microorganism can grow on economically and environmentally sustainable carbon sources, including:
• glucose, technical glycerol and crude glycerol;
• b. an organic component derived from the micro-organism;
• where said organic component includes at least one of the following:
• a biopolymer and a volatile substance, both protective to environmental stress, including cold stress;
• wherein the biopolymer produced and secreted in the presence of glycerol and proportional to the amount of glycerol present in the medium from the microorganism of the formulation, corresponds to a sugar polymer of high molecular weight similar to maltodextrin, with cryoprotective capacity in microorganisms and multicellular organisms, including bacteria and plants;
• and where the volatile substance has protective capacity for plants, with respect to adverse environmental conditions, including:
• water stress, low temperatures, freezing and pests, where said volatile substance is a stimulator of root growth and where in addition, said volatile substance corresponds to a mixture of volatile substances and where said mixture contains at least two of the elements of the list comprising:
• Dimethyl disulfide, 1-Nonene, 1-Undecanol, 2-Undecanone, 2-Pentane, 3-Methyl-Butanal, Methyldiselenide, 2-Heptane, 2-Nonanone, any other volatile substance produced by a microorganism of the composition and combinations thereof;
• c. a means of solubilizing the micro-organism and the organic component derived from the micro-organism;
• and
• d. a stabilizer.