US 12,721,282 B2 · Microbial composition for enhancing the inoculation rate of Tricholoma matsutake mycelium and use thereof

Abstract

There is provided a microbial composition comprising Paenibacillus cineris and Micrococcus sp. for enhancing the inoculation rate of T. matsutake mycelium in trees, as well as a use thereof. The microbial composition according to the present invention significantly increases the inoculation rate of T. matsutake mycelium in trees. Furthermore, the composition does not inhibit the growth of T. matsutake mycelium and effectively suppresses harmful microorganisms that interfere with mycelium growth. The microbial composition of the present invention also remarkably improves the survival rate of T. matsutake-inoculated seedlings. According to the method for producing T. matsutake mycelium-inoculated seedlings of the present invention, seedlings with a high inoculation rate of T. matsutake mycelium and a high survival rate can be mass-produced and supplied in large quantities to mushroom cultivators.

Claims5

Dependent claims

A method for producing T. matsutake mycelium-inoculated seedlings, comprising the steps of:

i) preparing a T. matsutake mycelium culture solution by liquid culturing the T. matsutake mycelium and homogenizing the culture using a homogenizer;
ii) culturing Paenibacillus cineris and Micrococcus sp. and centrifuging the cultures to obtain microbial pellets;
iii) washing and pruning the roots of pine seedlings;
iv) immersing the roots of the root-pruned pine seedlings in a mixed solution prepared by mixing the T. matsutake mycelium culture solution of step (i) and the microbial pellets of step (ii) to inoculate the seedlings;
and
v) planting the inoculated pine seedlings in soil and cultivating them.

3 more elements

Dependencies are inferred from the claim text.

Microbial composition for enhancing the inoculation rate of Tricholoma matsutake mycelium and use thereof

Life of this patent

Microbial composition for enhancing the inoculation rate of Tricholoma matsutake mycelium and use thereof

Life of this patent

Abstract

Claims5