Mass spectrometer calibration
AssigneeDH Technologies Development Pte. Ltd.
InventorNicholas G. Bloomfield
Life of this patent
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Abstract
In one aspect, systems and method are provided for calibrating a hybrid mass spectrometer. The calibration may include, for instance, an accurate mass calibration and a nominal mass calibration performed across a plurality of transmission widths and scanning speeds applied for each reference standard, ideally each reference standard representing a different m/z, to produce a matrix of correction factors corresponding to each of the transmission width and scanning speed pairs at the m/z values for the reference standards evaluated. A multi-parameter interpolation may be applied to identify a correction factor to be used for a subsequent analysis transmission width and scanning speed pair that differs from the plurality of transmission widths and scanning speeds used to produce the correction factors.
Claims19
A method for calibrating a hybrid mass spectrometer comprising an accurate mass analyzer combined with a nominal mass analyzer, the method comprising:
- calibrating the accurate mass analyzer using one or more reference standards,
- analyzing a sample using the nominal mass analyzer and the accurate mass analyzer to generate one or more corresponding nominal and calibrated accurate mass signals associated with the sample, and
- calibrating the nominal mass analyzer by comparing the nominal and the calibrated accurate mass signals,
- wherein said nominal mass analyzer is configured to provide a plurality of ion transmission windows.
A method for calibrating a mass spectrometer having at least a first and a second mass analyzer, wherein the second mass analyzer is positioned downstream of the first mass analyzer, comprising:
- calibrating said second mass analyzer using one or more reference standards,
- generating a first measurement of a mass signal of a precursor ion using the first mass analyzer and generating a second measurement of a respective mass signal of said precursor ion using the second mass analyzer, and
- calibrating said first mass analyzer via a comparison of said first and second measurements of said mass signal,
- wherein said first mass analyzer is configured to provide a plurality of precursor ion transmission windows.
A hybrid mass spectrometer, comprising:
- a nominal mass analyzer configured to provide a plurality of transmission windows for passage of at least one precursor ion,
- a collision cell positioned downstream of said nominal mass analyzer for receiving said at least one precursor ion and causing fragmentation thereof so as to generate a plurality of product ions,
- an accurate mass analyzer for receiving ions exiting said collision cell and generating a mass spectrum thereof,
- a mass analyzer for receiving one or more operating parameters of said nominal mass analyzer and said mass spectrum generated by said accurate mass analyzer,
- wherein said mass analyzer is configured to identify a mass signal associated with said precursor ion in said accurate mass analyzer and calibrate said nominal mass analyzer based on an m/z ratio of said precursor ion in said mass spectrum and said one or more operating parameters of said nominal mass analyzer.
Dependencies are inferred from the claim text.