US 12,716,864 B1 · Organic electrochemical transistor as an on-site signal amplifier for electrochemical aptamer-based sensors

Abstract

Analyte sensing devices that include an electrochemical aptamer-based (E-AB) sensor integrated with an organic electrochemical transistor (OECT) for signal amplification are provided. The E-AB sensor has a three-electrode setup that includes an aptamer-functionalized working electrode, an independent reference electrode, and a counter electrode composed of a mixed ionic-electronic conductive polymer. In the sensors, the working electrode simultaneously serves as a gate electrode for an OECT, while the mixed ionic-electronic conductive polymer also acts as the transistor channel of the OECT. This integrated device design retains the features of both the E-AB sensor and the OECT, thereby enabling electrochemical measurements to be carried out by the E-AB sensor, while simultaneously monitoring changes in the in-plane conductivity of the OECT channel.

Claims20

Dependent claims

A referenced-organic electrochemical transistor (OECT)-based electrochemical aptamer-based (E-AB) sensor comprising:

an E-AB sensor comprising:
a working electrode comprising a redox-reporter-modified aptamer-functionalized electrode;
a reference electrode connected to the working electrode;
a counter electrode comprising a layer of a mixed ionic-electronic conductive polymer;
a voltage source for applying a voltage to the working electrode;
and
a working-electrode-current-detector for measuring a Faradaic current in the working electrode;
and
an OECT comprising:
a gate electrode comprising the redox-reporter-modified aptamer-functionalized electrode that is also the working electrode in the E-AB sensor;
a source electrode;
a drain electrode;
a channel connecting the source electrode to the drain electrode, the channel comprising the layer of the mixed ionic-electronic conductive polymer that is also the counter electrode in the E-AB sensor;
and
a channel-current-detector for measuring an in-plane current in the channel between the source electrode and the drain electrode.

12 more elements

A method of detecting an analyte in an electrolyte solution using a referenced-organic electrochemical transistor (OECT)-based electrochemical aptamer-based (E-AB) sensor comprising:

an E-AB sensor comprising:
a working electrode comprising a redox-reporter-modified aptamer-functionalized electrode;
a reference electrode connected to the working electrode;
a counter electrode comprising a layer of a mixed ionic-electronic conductive polymer;
a voltage source for applying a voltage to the working electrode;
and
a working-electrode-current-detector for measuring a Faradaic current in the working electrode;
and
an OECT comprising:
a gate electrode comprising the redox-reporter-modified aptamer-functionalized electrode that is also the working electrode in the E-AB sensor;
a source electrode;
a drain electrode;
a channel connecting the source electrode to the drain electrode, the channel comprising the layer of the mixed ionic-electronic conductive polymer that is also the counter electrode in the E-AB sensor;
and
a channel-current-detector for measuring an in-plane current in the channel between the source electrode and the drain electrode, the method comprising:
applying a time-varying potential to the working electrode;
measuring a Faradaic current in the working electrode as a function of the time-varying potential;
and
simultaneously measuring an in-plane current in the channel.

16 more elements

Dependencies are inferred from the claim text.

Organic electrochemical transistor as an on-site signal amplifier for electrochemical aptamer-based sensors

Life of this patent

Organic electrochemical transistor as an on-site signal amplifier for electrochemical aptamer-based sensors

Life of this patent

Abstract

Claims20