Method for mapping the surface of a macromolecule
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorStefan W. Hell
Life of this patent
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Abstract
In order to map the surface of a macromolecule, at least one fluorescent probe is introduced into a medium in which the macromolecule is embedded or will be embedded. Then, a plurality of spatial positions of the at least one fluorescent probe with regard to the macromolecule are determined via localization of the at least one singularized fluorescent probe with a simple standard deviation of no more than 2 nm. For this purpose, fluorescence light photons emitted by the singularized fluorescent probe are recorded. In addition, a bounding surface bounding the determined spatial positions with regard to the macromolecule is determined; and a three-dimensional map of at least a part of the surface of the macromolecule is generated from the bounding surface.
Claims20
A method for mapping the surface of a macromolecule, the method comprising the steps of:
- introducing at least one fluorescent probe into a medium in which the macromolecule is embedded or will be embedded;
- determining a plurality of spatial positions of the at least one fluorescent probe with regard to the macromolecule by localization of the at least one singularized fluorescent probe at a single standard deviation of not more than 2 nm, wherein fluorescence light photons emitted by the singularized fluorescent probe are registered;
- determining a bounding surface bounding the determined spatial positions with regard to the macromolecule;
- and
- generating a three-dimensional map of at least a part of the surface of the macromolecule from the bounding surface.
Dependencies are inferred from the claim text.