Signal processing method, signal processing device, and signal processing system
AssigneeHamamatsu Photonics K.K.
InventorsKenta Tsujii, Masato Asai, Koji Takemiya, Yoshiaki Yamauchi
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Abstract
The data processing device is a signal processing device that processes outputs of photomultiplier tubes configuring a flow cytometer system and includes a processor, in which the processor is configured to acquire intensity signals of the photomultiplier tubes based on signal light generated by flow cytometry using the flow cytometer system, calculate a virtual photon quantity of the signal light incident on the photomultiplier tubes by dividing a digital value of the intensity signal by a gain of the photomultiplier tubes and signal light quantum efficiency, and execute data analysis based on the virtual photon quantity of the signal light.
Claims17
A signal processing method for processing an output of a photomultiplier tube configuring a flow cytometer, the signal processing method comprising:
- acquiring an output current signal of the photomultiplier tube based on signal light generated by flow cytometry using the flow cytometer as a first current signal;
- calculating an analysis evaluation value that is either a photon quantity of the signal light incident on the photomultiplier tube or a photoelectron quantity emitted from a photoelectric conversion unit of the photomultiplier tube by dividing a value of the first current signal by a gain of the photomultiplier tube;
- executing data analysis based on the analysis evaluation value;
- and
- further calculating a first noise value corresponding to noise included in the analysis evaluation value based on the analysis evaluation value and the gain of the photomultiplier tube, wherein the data analysis includes gating processing in which a section of a gate is set using the analysis evaluation value and the first noise value.
A signal processing method for processing an output of a photomultiplier tube configuring a flow cytometer, the signal processing method comprising:
- acquiring an output current signal of the photomultiplier tube based on signal light generated by flow cytometry using the flow cytometer as a first current signal;
- calculating an analysis evaluation value that is either a photon quantity of the signal light incident on the photomultiplier tube or a photoelectron quantity emitted from a photoelectric conversion unit of the photomultiplier tube by dividing a value of the first current signal by a gain of the photomultiplier tube;
- further calculating a first noise value including:
- own independent system noise caused by own signal light reflecting an expectation value of signal light to be detected in the photomultiplier tube and a gain of the photomultiplier tube;
- another independent system noise caused by another signal light reflecting an expectation value of signal light leaking into the photomultiplier tube out of signal light to be detected in another photomultiplier tube different from the photomultiplier tube and the gain of the photomultiplier tube;
- and own correlation system noise caused by a correlation between the own signal light and the another signal light reflecting the expectation value of the signal light to be detected in the photomultiplier tube and the gain of the photomultiplier tube;
- and
- executing data analysis using the analysis evaluation value and the first noise value.
A signal processing device that processes an output of a photomultiplier tube configuring a flow cytometer and includes a processor, wherein
- the processor is configured to:
- acquire an output current signal of the photomultiplier tube based on signal light generated by flow cytometry using the flow cytometer as a first current signal,
- calculate an analysis evaluation value that is either a photon quantity of the signal light incident on the photomultiplier tube or a photoelectron quantity emitted from a photoelectric conversion unit of the photomultiplier tube by dividing a value of the first current signal by a gain of the photomultiplier tube,
- execute data analysis based on the analysis evaluation value, and
- further calculate a first noise value corresponding to noise included in the analysis evaluation value based on the analysis evaluation value and the gain of the photomultiplier tube, wherein the data analysis includes gating processing in which a section of a gate is set using the analysis evaluation value and the first noise value.
A signal processing device that processes an output of a photomultiplier tube configuring a flow cytometer and includes a processor, wherein
- the processor is configured to:
- acquire an output current signal of the photomultiplier tube based on signal light generated by flow cytometry using the flow cytometer as a first current signal,
- calculate an analysis evaluation value that is either a photon quantity of the signal light incident on the photomultiplier tube or a photoelectron quantity emitted from a photoelectric conversion unit of the photomultiplier tube by dividing a value of the first current signal by a gain of the photomultiplier tube,
- further calculate a first noise value including:
- own independent system noise caused by own signal light reflecting an expectation value of signal light to be detected in the photomultiplier tube and a gain of the photomultiplier tube;
- another independent system noise caused by another signal light reflecting an expectation value of signal light leaking into the photomultiplier tube out of signal light to be detected in another photomultiplier tube different from the photomultiplier tube and the gain of the photomultiplier tube;
- and own correlation system noise caused by a correlation between the own signal light and the another signal light reflecting the expectation value of the signal light to be detected in the photomultiplier tube and the gain of the photomultiplier tube, and
- execute data analysis using the analysis evaluation value and the first noise value.
Dependencies are inferred from the claim text.