Method for carrying out real-time PCR

A method for performing an amplification process for amplification of nucleic acids, comprising:

• amplifying sample nucleic acids and reference nucleic acids in separate reaction preparations, the amplifying including amplifying a whole genome and concomitantly running at least one first comparative sample having a defined concentration of nucleic acid of a reference genome;
• detecting signals corresponding to the amplification in real-time;
• and
• during the amplification process, automatically adjusting, with a control unit, a number of amplification cycles and/or a duration of the amplification process based on the detected signals,
• wherein the detected signals corresponding to the amplification are based on detection of light emitted by fluorescent dyes intercalated into double-stranded DNA,
• wherein the automatic adjusting further comprises:
• identifying a first checkpoint when the detected signals indicate amplification of both the sample nucleic acids and the reference genome in the at least one first comparative sample;
• and
• after the identifying of the first checkpoint:
• comparing intensity of the sample nucleic acids and a second comparative sample comprising a quantitative reference that contains a desired amount of product in the whole genome application and no amplification enzyme;
• and
• terminating the amplifying of the sample nucleic acids and reference nucleic acids in response to the intensity of the sample nucleic acids and the quantitative reference being equal.