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US 12,716,080 B2Granted
Engineered phospholipase D mutants, methods of making engineered phospholipase D mutants, and uses thereof
AssigneeCornell University
InventorsJeremy Baskin, Reika Tei
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Abstract
Engineered phospholipase D mutants are described herein. Also described herein are methods of making engineered phospholipase D mutants. Additionally, methods of using engineered phospholipase D mutants are described.
Claims26
A mutant Phospholipase D (PLD) enzyme comprising an amino acid sequence that varies from the amino acid sequence of a wild type PLD enzyme as set forth in SEQ ID NO:
- 1 in 6 to 10 substitutions;
- wherein one of the substitutions is G381V;
- wherein the additional substitutions are selected from the group consisting of S3T, Q13H, K34R, P42L, P42S, P42T, K57R, A59V, M71I, I130M, K133R, F163L, S185G, S198F, D201G, D201N, F235L, G242V, P245A, H248Y, K249R, A258T, P263S, V264I, K275R, K327R, G328S, E331K, N338Y, P345S, T395I, G406S, T412N, G429D, Q439K, T450A, D480G, D480E, Q492R, A495V, T496I, and C504Y;
- and
- wherein the mutant PLD enzyme exhibits a transphosphatidylation activity at least 2-fold higher as compared to the wild type PLD enzyme.
2 more elements
Dependencies are inferred from the claim text.