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US 12,716,079 B2Granted
Process control for 3-hydroxypropionic acid production by engineered strains of aspergillus niger
AssigneeBattelle Memorial Institute
InventorsKyle R. Pomraning, Ziyu Dai, Jon K. Magnuson, Beth A Hofstad, Jeffrey J. Czajka, Joonhoon Kim, Shuang Deng
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Abstract
Provided herein are recombinant Aspergillus niger capable of producing 3-hydroxypropionic acid (3-HP). Also provided are methods of producing 3-hydroxypropionic acid (3-HP) and related kits.
Claims19
A recombinant Aspergillus niger, comprising an exogenous nucleic acid molecule encoding aspartate 1-decarboxylase (PAND), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH), wherein:
- i) the exogenous nucleic acid molecule encoding PAND encodes an amino acid sequence comprising at least 95% sequence identity to SEQ ID NO:
- 1;
- ii) the exogenous nucleic acid molecule encoding BAPAT encodes an amino acid sequence comprising at least 95% sequence identity to SEQ ID NO:
- 2;
- and
- iii) the exogenous nucleic acid molecule encoding HPDH encodes an amino acid sequence comprising at least 95% sequence identity to SEQ ID NO:
- 3.
4 more elements
Dependencies are inferred from the claim text.