Compositions for transfecting a nucleic acid molecule into a cell comprising benzo-fused heterocyclic compounds grafted to a cationic polymer, and their applications

A composition for transfecting a nucleic acid molecule into a cell, comprising (i) at least one compound of general formula (II), or an acceptable salt thereof, and (ii) an acceptable excipient, buffering agent, cell culture medium, or transfection medium:

• wherein:
• (i) Y1 and Y3 represent N, Y2 represents C;
• Z1 represents X1—R3—X2—P+;
• Z2 represents H, a linear or branched, saturated or unsaturated C1-C18 alkyl, CF3, a linear or branched, saturated or unsaturated C2-C18 heteroalkyl, C6-C18 aryl, a linear or branched, saturated or unsaturated C1-C18 alkylamine, a linear or branched, saturated or unsaturated C1-C18 alkyl-C1-C12 alkoxy, C1-C18 alkyl-halogen;
• Z3 is absent;
• Z4 represents H, or a linear or branched, saturated or unsaturated C1-C18 alkyl;
• Z5 represents H, a linear or branched, saturated or unsaturated C1-C18 alkyl, a C1-C12 alkoxy, halogen, OH, or carboxyphenyl;
• Z6 represents H, or a linear or branched, saturated or unsaturated C1-C18 alkyl;
• and Z7 represents H;
• or
• (ii) Y1, Y2 and Y3 represent N;
• Z1 represents X1—R3—X2—P+, or Z1 is absent;
• Z2 represents X1—R3—X2—P+, or Z2 is absent;
• with the proviso that only one of Z1 or Z2 represents X1—R3—X2—P+;
• Z3 is absent;
• Z4 represents H;
• Z5 represents H, a linear or branched, saturated or unsaturated C1-C18 alkyl, or a C1-C12 alkoxy;
• Z6 represents H, a linear or branched, saturated or unsaturated C1-C18 alkyl, or a C1-C12 alkoxy;
• Z7 represents H;
• X1 represents CH2;
• X2 represents CO;
• R3 represents (CH2)m, with m representing an integer between 1 and 3;
• P+ represents a graft cationic polymer, which is a linear or branched polyethyleneimine (PEI);
• R, T, U and V represent C.