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US 12,716,071 B2Granted
Methods for plant transformation
AssigneePioneer Hi-Bred International, Inc.
InventorsAjith Anand, William James Gordon-Kamm, George J Hoerster, Keith S Lowe, Kevin E McBride
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Abstract
The methods disclosed herein use a morphogenic gene or a repressor gene to provide a transgenic plant containing a single copy of a transgenic event wherein the morphogenic gene or the repressor gene does not integrate into the genome of the plant and the plant does not contain vector (plasmid) backbone.
Claims31
A method of producing a transgenic maize plant comprising:
- contacting a maize plant cell with a T-DNA containing a trait gene expression cassette and a T-DNA containing a morphogenic gene expression cassette;
- selecting a maize plant cell containing the trait gene expression cassette and no morphogenic gene expression cassette;
- and
- regenerating a transgenic maize plant from the selected plant cell,
- wherein the morphogenic gene cassette is not excised from the genome of the selected maize plant cell,
- wherein the contacting comprises bacteria-mediated transformation that is mediated by bacteria of a disarmed Agrobacteria strain and/or an Ochrobactrum haywardense H1 strain,
- wherein the contacting comprises contacting the maize plant cell with the T-DNA containing the trait gene expression cassette in a first group of bacteria and contacting the plant cell with the T-DNA containing the morphogenic gene expression cassette in a second, different group of bacteria, and
- wherein the morphogenic gene expression cassette comprises a nucleotide sequence encoding:
- (i) a Wuschel or Wuschel homeobox (WUS/WOX homeobox) polypeptide;
- (ii) a Babyboom (BBM) polypeptide or an Ovule Development Protein 2 (ODP2) polypeptide;
- or
- (iii) a combination of (i) and (ii).
9 more elements
Dependencies are inferred from the claim text.