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US 12,716,070 B2Granted
Dispersins expressed with amylase signal peptides
AssigneeNovozymes A/S
InventorMaria Tang
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Abstract
The present invention relates to nucleic acid constructs comprising a first polynucleotide encoding a signal peptide from a bacterial alpha-amylase a second polynucleotide encoding a polypeptide having hexosaminidase activity; expression vectors and host cells comprising said nucleic acid constructs; and methods for producing polypeptides having hexosaminidase activity.
Claims18
A nucleic acid construct comprising:
- a) a first polynucleotide encoding a signal peptide from a bacterial alpha-amylase, wherein the signal peptide has a sequence identity of at least 90% to SEQ ID NO:
- 2;
- and
- b) a second polynucleotide encoding a polypeptide having hexosaminidase activity, wherein the polypeptide having hexosaminidase activity belongs to the Terribacillus clade and comprises the motif [VIM][LIV]G[GAV]DE[VI][PSA] (SEQ ID NO:
- 11);
- wherein the signal peptide has an additional Ala at the C-terminus compared to SEQ ID NO:
- 2;
- wherein the polypeptide having hexosaminidase activity has a sequence identity of at least 95% to the mature polypeptide of SEQ ID NO:
- 6 or SEQ ID NO:
- 10;
- and
- wherein the first polynucleotide and the second polynucleotide are operably linked in translational fusion.
9 more elements
The bacterial host cell of 12, wherein the bacterial host cell is a Bacillus cell.
The bacterial host cell of 12, wherein the bacterial host cell is a Bacillus licheniformis cell.
Dependencies are inferred from the claim text.