Hydrodynamically controlled electric fields for high throughput transformation and high throughput parallel transformation platform

A method of performing cell transfection, the method comprising:

• flowing one or more mammalian cell suspension(s) through a plurality of flow paths, each flow path defined within a fluid transport structure, the flowing comprising causing, for a given flow path of the plurality of flow paths, a flow of a plurality of cells of the mammalian cell suspension through an axial cross-section of the given flow path concurrently, wherein the flow paths each comprise a minimum diameter of 50 μm, wherein the fluid transport structure comprises conductive elements configured to produce an electric field in the flow path, wherein the conductive elements are hollow cylindrical electrodes arranged axially at either end of the flow path;
• and
• applying a voltage to generate the electric field in the flow paths;
• wherein the electric field is sufficient to transfect the plurality of cells flowing through the corresponding axial cross-sections of the flow paths.