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US 12,716,060 B2Granted
CRISPR-associated systems and components
AssigneeArbor Biotechnologies, Inc.
InventorsDavid R. Cheng, David A. Scott, Winston X. Yan
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Abstract
The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered Type III-E CRISPR-Cas systems, components, and methods for targeted modification of DNA, RNA, and protein substrates. Each system includes one or more protein components and one or more nucleic acid components that together target DNA, RNA, or protein substrates.
Claims17
An engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas system comprising:
- an RNA guide comprising a direct repeat sequence and a spacer sequence capable of hybridizing to a target nucleic acid, or a nucleic acid encoding the RNA guide;
- and
- a CRISPR-associated protein, wherein the CRISPR-associated protein comprises an amino acid sequence that is at least 97% identical to the amino acid sequence of SEQ ID NO:
- 316, SEQ ID NO:
- 317, or SEQ ID NO:
- 318, or a nucleic acid encoding the CRISPR-associated protein;
- wherein the CRISPR-associated protein is capable of binding to the RNA guide and of targeting the target nucleic acid sequence complementary to the spacer sequence.
4 more elements
Dependencies are inferred from the claim text.