Immobilized proteases for activation of the zymogen form of transglutaminase
AssigneeCurie Co. Inc.
InventorsErika M. Milczek, Robert Dicosimo, Steven Walsh, Malcolm McCray
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Abstract
Immobilized proteases for activation of the zymogen form of transglutaminase are disclosed.
Claims3
A transglutaminase-activating M4 metalloprotease (TAMEP) and a tripeptidyl aminopeptidase (TAP) co-immobilized on a porous solid support, wherein higher transglutaminase activity is achieved when a microbial transglutaminase or variant thereof is activated by using the co-immobilized TAMEP and TAP compared to using the TAMEP and the TAP separately immobilized on the porous solid support, and wherein the TAMEP is from Streptomyces mobaraensis and the TAP is from Streptomyces mobaraensis.
A method for activating a zymogen form of a microbial transglutaminase or a variant thereof to produce a mature, catalytically active form of the microbial transglutaminase, the method comprising
- (a) co-immobilizing at least one transglutaminase-activating M4 metalloprotease (TAMEP) and at least one tripeptidyl aminopeptidase from (TAP) on the same porous solid support;
- and
- (b) contacting the zymogen form of the microbial transglutaminase with co-immobilized TAMEP and TAP to produce a mature, catalytically active form of the microbial transglutaminase;
- wherein the TAMEP is from Streptomyces mobaraensis and the TAP is from Streptomyces mobaraensis;
- and
- wherein the method results in higher transglutaminase activity compared to using the TAMEP and the TAP separately immobilized on the porous solid support to activate the zymogen form of the microbial transglutaminase or variant thereof.
Dependencies are inferred from the claim text.