Method for differentiating pluripotent stem cells into desired cell type
AssigneeKeio University
InventorMinoru Ko
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Abstract
Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.
Claims6
A method of differentiating a pluripotent stem cell into a hematopoietic stem cell and/or a blood cell, comprising a step of introducing only SPI1 as a transcription factor into a pluripotent stem cell of mammalian origin.
A method of differentiating a pluripotent stem cell into a hematopoietic stem cell and/or a blood cell, comprising a step of introducing one or more transcription factors of CDYL2, ETS2, CEBPB and SALL4 into a pluripotent stem cell of mammalian origin.
Dependencies are inferred from the claim text.