Protein hydrogel, preparation method and use thereof

A method of producing a protein hydrogel comprising reagent A, being gelatin, reagent B, being a cross-linking agent, being GTA, and solvent, the method comprising the steps of:

• a) addition a suitable amount of reagent A, in an aqueous solution;
• b) heating up the mixture of step a) to dissolve the gel;
• c) initially stabilizing the gel;
• d) preparing reagent B, by dissolving it in an aqueous solution and cooling it;
• e) adding reagent B, as prepared in step d), to the gel prepared in step c);
• f) cross-linking;
• characterised in that reagent A is present in the final concentration from 0.3% wt. to 0.8% wt., with a ratio of reagent A to reagent B of 0.75-2.4 mg to 0.021-0.045 mg respectively,
• the initial stabilization of the gel in step c) takes place when the gel reaches the temperature 0° C.-12° C. and its duration is at least about 5 minutes;
• steps d)-f) are performed at reduced temperature from about 0° C. to about 12° C.,
• in step f) the duration of cross-linking is at least 12 h.