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US 12,714,767 B2Granted
Hybrid-hydrogels comprising decellularized extracellular matrix
AssigneeThe Regents of the University of Colorado, a body corporate
InventorsChelsea Magin, Cassandra Petrou, Rukshika Shalani Hewawasam
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Abstract
The present invention relates in part to hybrid hydrogel scaffolds including a decellularized extracellular matrix (dECM) tissue, and a synthetic polymer. The dECM may include any suitable tissue including for example, lung tissue, heart tissue, heart-lung block tissue, skin tissue, liver tissue, pancreatic tissue, kidney tissue, and the like.
Claims20
A hybrid hydrogel scaffold comprising:
- a decellularized extracellular matrix (dECM) tissue comprising lung tissue, and
- a synthetic polymer crosslinked with the dECM,
- wherein the dECM is thiolated and
- wherein the synthetic polymer comprises alpha-methacrylate functionality and has a photo-tunable stiffness;
- and
- a molar excess of alpha-methacrylate functionality of the synthetic polymer to the thiol of the dECM.
3 more elements
A method for generating a hybrid hydrogel, the method comprising:
- preparing a thiolated dECM,
- preparing a synthetic polymer solution,
- chemically crosslinking the dECM and the synthetic polymer,
- swelling the crosslinked dECM and synthetic polymer using one or more swelling solutions, thereby generating a hydrogel,
- selectively photo-crosslinking the swelled hydrogel using a patterned mask,
- seeding a plurality of cells onto the photo-crosslinked hydrogel, and
- culturing the cells on the pattern-photo-crosslinked hydrogel.
4 more elements
Dependencies are inferred from the claim text.