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US 12,714,766 B2Granted
Method of in vitro chondrocyte and cartilage culture to obtain material for the treatment of articular cartilage defects
AssigneeArthec S.A.
InventorsSławomir Jaros, Grzegorz Sobieraj, Julita Balcerek
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Abstract
The present invention relates to a new method for treating human or animal tissue such as cartilage, particularly damaged tissue. More specifically, the invention relates to a method to obtain material for treating the damaged tissue, for example any cartilage damage, in particular as a result of traumatic or degenerative cartilage damage.
Claims18
A method for producing a cartilage tissue implant in vitro, the method comprising:
- i) providing at least a first and a second fragment of viable cartilage tissue, which each have at least one edge, wherein one edge of the first fragment is complementary in shape to one edge of the second fragment, wherein the first fragment and the second fragment of viable cartilage tissue comprise viable chondrocytes;
- ii) placing the fragments in a culture medium such that at least one edge of the first fragment is spaced 1 cm or less apart from at least one edge of the second fragment;
- iii) adding a suspension of viable chondrocytes to the fragments from step (ii);
- and
- iv) cultivating the fragments and chondrocytes under cell culture conditions to thereby produce a cartilage tissue implant in vitro,
- wherein the edges of the fragments are in contact with each other along a tangential section, and wherein the tangential section of contact has a length of at least 0.5 cm.
3 more elements
A method of repairing damaged hyaline cartilage tissue in a patient in need thereof comprising:
- i) obtaining at least a first and a second fragment of viable hyaline cartilage tissue from the patient, which each have at least one edge, wherein one edge of the first fragment is complementary in shape to one edge of the second fragment, wherein the first fragment and the second fragment of viable hyaline cartilage tissue comprise viable chondrocytes;
- ii) placing the fragments in a culture medium such that the edges of the fragments are in contact with each other along a tangential section, and wherein the tangential section of contact has a length of at least 0.1 cm;
- iii) adding a suspension of viable chondrocytes to the fragments from step (ii);
- iv) cultivating the fragments and chondrocytes under cell culture conditions to thereby produce a cartilage tissue implant in vitro;
- and
- v) repairing damaged hyaline cartilage tissue in the patient by implanting the cartilage tissue implant in the patient.
3 more elements
Dependencies are inferred from the claim text.