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US 12,714,751 B2Granted
Light-induced cellular production of immune functional extracellular vesicles
AssigneeUniversity of Florida Research Foundation, Inc.
InventorMei He
Life of this patent
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Abstract
Described are method of producing extracellular vesicles from cells by exposing the cells to near UV light. Compositions containing the extracellular vesicles and methods of using the extracellular vesicles are also described.
Claims20
A method for producing extracellular vesicles comprising:
- exposing at least one cell to near UV-visible light, wherein intensity of light exposure on the cells is about 0.05 W/cm2 to about 0.25 W/cm2, wherein exposing the at least one cell to near UV-light increases production of extracellular vesicles from the at least one cell.
A method of providing therapeutic extracellular vesicles to a subject comprising:
- exposing at least one cell to near UV light to induce production of extracellular vesicles;
- isolating the extracellular vesicles;
- optionally loading the extracellular vesicles with one or more therapeutic molecules, and administering the extracellular vesicles to the subject.
A method of immunotherapy comprising:
- exposing at least one cell containing or expressing a therapeutic polypeptide or nucleic acid to near UV light to induce production of extracellular vesicles;
- isolating the extracellular vesicles;
- optionally loading the extracellular vesicles with one or more therapeutic molecules, and administering the extracellular vesicles to the subject.
Dependencies are inferred from the claim text.