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AssigneeHangzhou Grand Biologic Pharmaceutical Inc.
InventorsRiyong Liu, Zhiming Wang, Yunfeng He, Yu Wang, Zhicheng Fu, Tianwen Yan, Chunlan Hu, Guowei Su, Changcheng Tan, Xupeng Ding, Hui Yang, Hongying Wang, Qiong Ding, Qian Wang, Haiyan Wen, Kai Fan
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Provided is a method for preparing a polyethylene glycol-modified urate oxidase, at least 11 of the following amino acid sites of the polyethylene glycol-modified urate oxidase have a PEG modification: T1, K3, K4, K30, K35, K76, K79, K97, K112, K116, K120, K152, K179, K222, K231, K266, K272, K285, K291, and K293, and the preparation method includes: performing a coupling reaction between urate oxidase and polyethylene glycol, wherein the polyethylene glycol is provided in the form of an acidic solution, and a molar ratio of the urate oxidase to the polyethylene glycol is 1: (56 to 94), to obtain the polyethylene glycol-modified urate oxidase.
A method for preparing a polyethylene glycol-modified urate oxidase, characterized by performing a coupling reaction between urate oxidase and polyethylene glycol to obtain the polyethylene glycol-modified urate oxidase, wherein the polyethylene glycol is provided in a form of an acidic solution, and a molar ratio of the urate oxidase to the polyethylene glycol is 1:
Dependencies are inferred from the claim text.