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US 12,714,737 B2Granted
Recombinant proteins, compositions and methods of stabilization thereof
AssigneeUnichem Laboratories Ltd
InventorsDhananjay Sathe, Vivek Mishra, Saravanakumar Iyappan, Sunil Jog
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Abstract
The present invention relates to recombinant proteins, compositions and methods of stabilization thereof. The invention specifically relates to the stable recombinant protein of SEQ ID NO: 1 and composition comprising recombinant protein of SEQ ID NO: 1. The invention further relates to method of preparing, storing and stabilizing recombinant protein of SEQ ID NO: 1 and its composition for longer shelf life.
Claims4
A method for stabilizing a composition comprising a recombinant lectin protein of SEQ ID NO:
- 1 and stabilizing agent(s) during storage or exposure to light, comprising:
- a. introducing the composition into a container to obtain a stabilized composition, wherein
- i. the container resists light waves of wavelength between 100 nm and 500 nm;
- ii. a headspace of the container comprises inert atmosphere or vacuum;
- and
- iii. the stabilizing agent(s) comprises an amino acid or its salt;
- a surfactant;
- a sugar or carbohydrate;
- or a combination thereof;
- wherein the stabilized composition exhibits less than 5% of high molecular weight impurities or related substance impurities individually or in combination, optionally, when the composition is stored for a period of 6 months at a temperature ranging from 20° C.-25° C. and relative humidity of 60% or when the composition is stored for a period of 1 year at 2° C.-8° C.
7 more elements
Dependencies are inferred from the claim text.