Production of a probiotic composition
AssigneesBluestone Pharma GmbH, Blis Technologies Limited
InventorsElisabeth Kirl, Bernhard Hofbauer, Michael Faber, Karsten Brandt
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Abstract
Provided is a method of producing a freeze-dried probiotic composition as well as a method of producing a probiotic raw material which involves culturing of a Streptococcus salivarius strain. Furthermore, a corresponding probiotic composition and a corresponding probiotic raw material is provided.
Claims25
A method of producing a probiotic raw material, wherein the method comprises at least the following steps:
- (i) cultivating Streptococcus salivarius in a growth medium comprising dextrose as a carbon source at a concentration of about 25 to 40 g/L when cultivation begins, wherein the growth medium has a pH of 6.8 to 7.2;
- (ii) lowering the pH to pH 5 to 5.5 by adding a pH lowering agent when the concentration of dextrose reaches 10-15 g/L and raising the pH after 30 minutes to 3 hours to pH 6.8 to 7.2 until cultivation ends;
- and
- (iii) harvesting the Streptococcus salivarius.
A method of cultivating Streptococcus salivarius, wherein the method comprises at least the following steps:
- (i) cultivating Streptococcus salivarius in a growth medium comprising dextrose as a carbon source at a concentration of about 25 to 40 g/L when cultivation begins, wherein the growth medium has a pH 6.8 to 7.2;
- and
- (ii) lowering the pH to pH 5 to 5.5 by adding a pH lowering agent when the concentration of dextrose reaches 10-15 g/L and raising the pH after 30 minutes to 3 hours to pH 6.8 to 7.2 until cultivation ends.
A method for producing a probiotic composition comprising Streptococcus salivarius, or for producing a probiotic raw material comprising Streptococcus salivarius, using an allergen-free medium which does not comprise a milk product, the method comprising:
- (i) cultivating Streptococcus salivarius in a growth medium comprising yeast extract and dextrose, wherein dextrose is present at a concentration of about 25 to 40 g/L when cultivation begins, wherein the growth medium has a pH of 6.8 to 7.2;
- and
- (ii) lowering the pH to pH 5 to 5.5 by adding a pH lowering agent when the concentration of dextrose reaches 10-15 g/L and raising the pH after 30 minutes to 3 hours to pH 6.8 to 7.2 until cultivation ends.
Dependencies are inferred from the claim text.