Formulations and methods for treating diarrhea

A formulation for use in treating diarrhea in a subject in need thereof, wherein the formulation comprises a therapeutically effective combination of free amino acids:

• the free amino acids consisting essentially of a therapeutically effective amount of free amino acids of proline and aspartic acid;
• and
• a therapeutically effective amount of at least one of free amino acids of serine, threonine, glycine, alanine, arginine, or tyrosine, or any combination thereof;
• and
• optionally, free amino acids of asparagine;
• or
• optionally,
• monosaccharide glucose,
• at least one glucose-containing disaccharide, or
• any combination thereof,
• wherein the total concentration of the monosaccharide glucose, the at least one glucose-containing disaccharide, or any combination thereof is equal to or less than 90 mM;
• or
• optionally, at least one pharmaceutically acceptable carrier, buffer, electrolyte, adjuvant, excipient, or water, or any combination thereof;
• or
• any combination thereof;
• and
• wherein the therapeutically effective combination of free amino acids is sufficient to reduce at least one symptom associated with the diarrhea in the subject.