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US 12,710,434 B2Granted
Red cell diluent with chelating agent and methods for making and using the same
AssigneeImmucor, Inc.
InventorsMortimer Alzona, Bryan Marshall, Elizabeth Cope, Margot Borgel, Teresa Welch
Life of this patent
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Abstract
The present disclosure relates to a RBC solution comprising ethylenediaminetetraacetic acid (EDTA) and methods for making and using the same. The solution can comprise EDTA at a concentration from 3-5 g/L, including 4 g/L. Well plates prepared using the solution can provide a decreased rate of false positive results in automated immunoassays for detecting RBC antibodies in a patient sample.
Claims15
A method comprising:
- preparing a red blood cell (RBC) solution comprising RBCs and a chelating agent, the RBCs comprising antigens to antibodies to be identified in a patient sample;
- applying the RBC solution to a structure to cause the RBCs in the solution to be adhered to a surface of the structure;
- and
- applying a drying solution to the structure having the RBCs or fragments of the RBCs adhered to the surface of the structure.
1 more element
Dependencies are inferred from the claim text.