LAMA2, PLXDC2 and MLL4 as novel biomarkers for prediabetes and diabetes

A lateral flow diagnostic device for detecting and identifying prediabetes and/or diabetes, comprising:

• (i) a substrate having a top surface and a bottom surface opposite to the top surface, and a top end and a bottom end opposite to the top end;
• (ii) a sample loading area;
• (iii) a capture antibody area, containing capture antibodies immobilized to the substrate in the capture antibody area to capture prediabetes and diabetes protein markers consisting of MLL4, LAMA2 and PLXDC2;
• (iv) a reagent area, containing a conditioning reagent;
• (v) a detection antibody area, containing detection antibodies to detect the captured prediabetes and diabetes protein markers consisting of the MLL4, LAMA2 and PLXDC2;
• and
• (vi) optionally a positive control area;
• wherein the sample loading area, the detection antibody area, the reagent area, the capture antibody area, and the positive control area are located on the top surface of the substrate, allowing these areas to be in fluidic communication, the sample loading area being located at the top end and the capture antibody area located at the bottom end with the optionally positive control area located either after or before the capture antibody area.